Pretty much all you can distinguish at 400x are the plasmalemma and nucleus and cytoplasm. If you have prepared slides of cell divisions, then you are able to see the individual chromosomes quite well. Most microscopes you can buy at Toy R Us are 10x eyepiece with 4x, 10x, and 40x lenses. This was all seen in 400X total magnification. The depth of field is now small and you … As you can see, magnification is never an issue for you. 2. More magnification will not make it better. In fact, more magnification always means the image is more blurry, not more crisp - it's always a compromise between size and blurriness that decides the optimal magnification… 400x: This magnification is useful for looking inside cells. Select the 400x magnification. You can do this in two ways: (1) scope’s aperture in inches multiplied by 50 or (2) focal length of aperture in millimeters times two. The mitochondria and lysosomes and others may be visible as "granules" in the cytoplasm, Sometimes if there is a lot of ER or Golgi, some stains will show them as just darkly stained regions, but you can… In contrast, the minimum magnification … 3. The individual chambers you see are cells, the smallest functional unit of an organism. The stage that the cell is currently in is prophase. Also, the cell walls in the onion root were barely visible, but the nuclei were very clear. If necessary, adjust the fine focus. You should be able to make out that sperm is present with 100x (10x X 10x), and should be able to see individual sperm at 400x… Now, what do you see? Aside from that, you can also compute for the theoretical maximum magnification of your scope. In one particular cell’s nucleus, the chromatin has condensed so much that it can be seen using a light microscope.